Student Reflection - Amanda Means

The role of B56d/g and B56g/g in Wnt signaling

Through the opportunity provided to me by FAST, I have been able to accomplish important research goals over the course of this summer. Since our lab focuses on the use of Xenopus laevis, or African Clawed Frogs, there is a large amount of time that has to be put into keeping those frogs healthy and viable for research. During an average semester, it is much more difficult to do research around this necessary frog care. Having the opportunity to spend more time working in the lab over the summer has allowed me to learn important techniques that are essential in this area of research.

One of the main goals I accomplished during this time is learning to microinject RNA into embryos. We are studying a protein which is a part of a pathway, or chain of reactions, that is key in proper development. Injection of RNA, a template from which proteins are made, into an embryo in early stages of development allows us to see how that protein might function in  development. In order to become proficient in microinjection, one must learn how to make and calibrate needles, be able prepare precise concentrations and volumes of RNA, and become used to working while looking through a microscope. During the summer I have been able to practice all of these. My other role in this research is as the frog surgeon. In order to prepare the Xenopus embryos, we perform in vitro fertilization, which requires the removal of testes from the male frogs via survival surgeries. Through practice, I have gained more confidence suturing and treating the frogs.

As it is my goal to become a surgeon, I have gained important skills and experience that will help me in my career path during my summer research. As a pre-medical student, it is vital that I learn how to work in a group setting. The opportunity provided by FAST has allowed me time to research apart from the stress of dealing with homework and classes.