A Method of Repeated Sampling of Static Headspace Above Anaerobic Bacterial Cultures

Verena Stalder, Nicole Bernard, Kurt W. Hanselmann, Reinhard Bachofen, and Thomas G. Chasteen(*)

Institute for Plant Biology and Microbiology, University of Zuerich, Zollikerstrasse 107, CH-8008 Zuerich, Switzerland and (*) Department of Chemistry, Sam Houston State University, Huntsville, Texas USA

Analytica Chimica Acta, 1995, Vol. 303, pages 91-97. ACA abstract


An enclosure device has been built that will allow for the long-term sampling of headspace gases and solution from anaerobic bacterial cultures. Specific design characteristics allow for a strict anaerobic environment to be maintained for long periods of time while the piercable rubber septa are isolated from the culture headspace to prevent interaction of the septa with reactive culture components. Using capillary gas chromatography coupled to fluorine-induced chemiluminescence detection, an oxygen intolerant, selenium resistant bacterial culture was grown and sampled repeatedly over 240 hours. The results show that this system is a useful and reliable tool for time course experiments with anaerobic bacterial cultures as well as with reactive, oxygen sensitive or extremely volatile substances.
A chromatogram from one of the experiments from this research is available.
Time course data is also here. For more information on how a dynamic headspace sampling device can be applied to bioremediating bacterial cultures see McCarty et al. paper from this research group.
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