Donovan C. Haines

Assistant Professor

Department of Chemistry

Department of Chemistry
Sam Houston State University
Huntsville, TX 77341

Office: CFS 317

Phone: (936) 294-1530
Fax: (936) 294-4996
Email: haines@shsu.edu

Experience

Fields of Research and Teaching Specialization

Courses (Previously at University of Texas at Dallas)

 

Research

My research involves the structure and mechanism of action of monooxygenase systems, especially those involved in metabolism of natural products, neurotransmitters, drugs, and hormones. This fundamental research has direct medical relevance as aberrant behavior of oxygen utilizing enzymes has been implicated to varying degrees in many neurodegenerative diseases including Amyotrophic Lateral Sclerosis (Lou Gehrig's disease), Parkinson's disease, and Alzheimer's disease. To study these enzymes my laboratory employs organic synthesis to produce enzyme inhibitors, biochemistry to measure the effects of the inhibitors and probe enzyme mechanism, molecular biology to probe the effects of changes in protein sequence on enzyme properties, and biophysics via spectroscopy and crystallography to study the structure and physical properties of the enzymes. In addition to using small molecules to study enzymes, we also use enzymes to synthesize and study interesting small molecules on a research (200 mg) scale. Ongoing projects in the lab include the characterization cytochrome P450 mediated quorum quenching and the P450 mediated 24S-hydroxylation of cholesterol.

For more information, visit my Lab Home Page.

Publications

If you have a subscription, you can access my entry in SCOPUS.

Chowdhary, P.K, Stewart, L., Lopez, C., and Haines , D.C. , "A Single Mutation in P450BM-3 Enhances Acyl Homoserine Lactone : Acyl Homoserine Substrate Binding Selectivity Nearly 250-Fold",  Journal of Biotechnology, in press, accepted May, 2008.

Teiber, J.F., Horke, S.,  Haines, D.C., Chowdhary, P.K., Xiao, J., Kramer, G.L., Haley, R.W. and Draganov, D.I., "Dominant Role of Paraoxonases in the Inactivation of the Pseudomonas aeruginosa Quorum Sensing Signal N-(3-Oxododecanoyl)-L-Homoserine Lactone",  Infection and Immunity, in press, accepted March, 2008.

Haines, D.C., Chen, B., Tomchick, D.R., Bondlela, M.,  Hegde, A., Machius, M., and Peterson, J.A., "Crystal Structure of Inhibitor Bound P450BM-3 Reveals Open Conformation of Substrate Access Channel",  Biochemistry, 47, 12, pp. 3662-70, 2008.

Chowdhary, P.K., Keshavan, N., Nguyen, H., Peterson, J.A., Gonzalez, J.E., and Haines, D.C., “Bacillus megaterium CYP102A1 Oxidation of Acyl Homoserine Lactones and Acyl Homoserines”, Biochemistry, 46, 50, pp. 14429-37, 2007.

Hegde, A., Haines, D.C., Bondlela, M., Chen, B., Schaffer, N., Tomchick, D.R., Machius, M., Nguyen, H., Chowdhary, P.K., Stewart, L., Lopez, C., and Peterson, J.A., "Interactions of Substrates at the Surface of P450s Can Greatly Enhance Substrate Potency", Biochemistry, 46, 49, pp. 14010-7, 2007.

Chowdhary, P.K, Alemseghed, M., and Haines, D.C., “Cloning, Expression, and Characterization of a New Self-Sufficient P450: CYP102A5 from Bacillus cereus”, Archives of Biochemistry and Biophysics, 468, 1, pp. 32-43, 2007.

Kitazume, T., Haines, D.C., Estabrook, R.W., Chen, B., and Peterson J.A., "Obligatory Intermolecular Electron Transfer from FAD to FMN in Dimeric P450BM-3", Biochemistry, 46, 42, pp. 11892-901, 2007.

Haines, D.C., " A Role for the Strained Phenylalanine Ring Rotation Induced by Substrate Binding to Cytochrome CYP102A1", Protein and Peptide Letters, 13, 10, pp. 977-80, 2006.

Keshavan, N.D., Chowdhary, P.K., Haines, D.C., and Gonzalez, J.E., "L-Canavanine Made By Alfalfa Interferes With Quorum Sensing in Sinorhizobium meliloti", Journal of Bacteriology, 187, 24, pp. 8427-36, 2005.

Kou, W., Kolla, H.S., Ortiz-Acevedo, A., Haines, D.C., Junker, M., and Dieckmann, G.R. , "Modulation of Zinc- and Cobalt-Binding Affinites Through Changes in the Stability of the Zinc Ribbon Protein L36" , Journal of Biological Inorganic Chemistry, 10, 2, pp. 167-80, 2005.

Wanduragala, S., Wimalasena, D.S., Haines, D.C., Kahol, P.K., and Wimalasena, K., , pH-Induced Alteration and Oxidative Destruction of Heme in Purified Chromaffin Granule Cytochrome b561: Implications for the Oxidative Stress in Catecholaminergic Neurons, Biochemistry, 42, n. 12, pp. 3617-26, 2003.

Wimalasena, D.S., Jayatillake, S.P., Haines, D.C., Wimalasena, K., Plausible Molecular Mechanism for Fumarate Activation and Electron Transfer of the Dopamine Beta-Monooxygenase Reaction, Biochemical Journal, v. 367, pt. 1, pp. 77-85, 2002.

Haines, D.C., Tomchick, D.R., Machius, M., and Peterson, J.A., The Pivotal Role of Water in the Mechanism of P450BM-3, Biochemistry, 40, n. 45, pp. 13456-65, 2001.

Falck, J.R., Reddy, Y.K, Haines, D.C., Reddy, K.M., Krishna, U.M., Graham, S., Murry, B., and Peterson, J.A., Practical, Enantiospecific Syntheses of 14,15-EET and Leukotoixn B (Vernolic Acid), Tetrahedron Letters, 42, 4131-3, 2001.

Haines, D.C., Sevrioukova, I.F., and Peterson, J.A., The FMN-Binding Domain of P450BM-3: Resolution, Reconstitution, and Flavin Analogue Substitution, Biochemistry, 39, n. 31, pp. 9419-29, 2000. 

Wimalasena, K., Wimalasena, D.S., Dharmasena, S., Haines, D.C., and Alliston, K.R., Chiral Multisubstrate Inhibitors of Dopamine b -Monooxygenase: Evidence for Dual Modes of Interaction, Biochemistry, 36, pp. 7144-53. 1997.

Wimalasena, K. and Haines, D.C., A General Progress Curve Method for the Kinetic Analysis of Suicide Enzyme Inhibitors, Analytical Biochemistry, 234, pp. 175-82, 1996.

Wimalasena, K. and Haines, D.C., Nucleophilic Substitution Reactions of Phenacyl Bromide Oxime: Effect of the Solvent and the Basicity of the Nucleophile, Journal of Organic Chemistry, 59, pp. 6472-4, 1994.
 

 

Last modified 05/16/2008 .