A Method of Repeated Sampling of Static Headspace Above Anaerobic Bacterial
Verena Stalder, Nicole Bernard, Kurt W. Hanselmann, Reinhard Bachofen,
and Thomas G. Chasteen(*)
Institute for Plant Biology and Microbiology, University of Zuerich, Zollikerstrasse
107, CH-8008 Zuerich, Switzerland and (*) Department of Chemistry, Sam
Houston State University, Huntsville, Texas USA
Analytica Chimica Acta, 1995, Vol. 303, pages 91-97. ACA
device has been built that will allow for the long-term sampling of
headspace gases and solution from anaerobic bacterial cultures. Specific
design characteristics allow for a strict anaerobic environment to be maintained
for long periods of time while the piercable rubber septa are isolated
from the culture headspace to prevent interaction of the septa with reactive
culture components. Using capillary gas chromatography coupled to fluorine-induced
chemiluminescence detection, an oxygen intolerant, selenium resistant bacterial
culture was grown and sampled repeatedly over 240 hours. The results show
that this system is a useful and reliable tool for time course experiments
with anaerobic bacterial cultures as well as with reactive, oxygen sensitive
or extremely volatile substances.
chromatogram from one of the experiments from this research is available.
course data is also here. For more information on how a dynamic
headspace sampling device can be applied to bioremediating bacterial
cultures see McCarty
et al. paper from this research group.
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