Determination of Method Detection
Limit of Tellurium in Sucrose Density Gradient Solutions by Inductively
Coupled Plasma Spectrometry
J. Pugh and T. G.
Department of Chemistry
Sam Houston State University
Huntsville, Texas 77341-2117
A 2.7 L bioreactor was inoculated with a facultative
anaerobe Pseudomonas fluorescens K27. The culture was amended with 10 mM
sodium tellurite. Seventy-two hours later, in the late stationary growth
phase, solid and liquid phases were separated by high speed centrifugation
and the solid resuspended and overlain on a solution consisting of several
layers of sucrose of varying concentrations. Following high speed centrifugation,
the sucrose density gradient allowed for the separation of bacterial cells
and elemental tellurium. Fractionated samples consisting of sucrose, 10%
nitric, and tellurium were analyzed using inductively coupled plasma (ICP).
The method detection limit (MDL) for tellurium in solutions was determined
in varying concentrations of sucrose.