Research activities have involved studies on bacterial endospores with emphasis on formation, activation, and germination. These studies have included endospores of mesophilic and thermophilic bacilli and thermophilic actinomycetes. These studies have been published in the Journal of Bacteriology, the Proceedings of Sixth, Seventh, and Eighth International Spore Conference, and the Archives of Microbiology, a publication of the German Society of Microbiology.
Studies on the endospores of Bacillus stearothermophilus NGB 101 have shown that these spores, like the spores of mesophilic bacilli, can be be high temperature activated, but in addition can be effectively activated at reduced temperatures. These are the first published results of low temperature activation of spores of the genus Bacillus. The ability to activate the spores of NGB 101 by shifting them to and holding them at temperatures of 30 degrees C offers the opportunity for studying the activation of bacterial spores uncomplicated by the effects of heat. A point of special interest is that recent studies have shown that low temperature activation of NGB 101 spores can be blocked by potassium cyanide, a powerful and highly specific inhibitor of cytochrome oxidase. The striking effect could mean that activation is dependent on energy transduction, thus having a physiological basis, and is currently under investigation.
Recent Selected Publications
The effects of alterations in the suspending medium on low temperature activation of spores of Bacillus stearothermophilus NGB 101. Archives of Microbiology. (1985) 142: 185-189.
Cloning and nucleotide sequencing of genes for small acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus and "Thermoactinomyces thalpophiles". (1986) 167: 168-173.