Analysis of Solid and Liquid
Phases of Biological Cultures Amended with Selenium and Tellurium Salts
J. Swearingen, Jr.
and T. G. Chasteen
Department of Chemistry
Sam Houston State University
Huntsville, Texas 77341-2117
Presented at the 58th ACS Southwest Regional Meeting in
Austin, Texas USA November 3-6, 2002.
The bacteria Pseudomonas fluorescens K27 were
simultaneously amended with soluble but toxic selenium and tellurium salts.
The bacterium reduced some of the soluble salts to the elemental form,
which are insoluble and were harvested from the cultures in late stationary
phase. Inductively coupled plasma atomic emission spectroscopy (ICP-AES)
was used in order to determine selenium and tellurium concentrations in
the solid and liquid extracts from biological cultures.